师资队伍

  • 姓名:武晓云
  • 性别:
  • 职称:副教授
  • 硕、博导:硕导
  • 学科领域:植物病理学
  • 研究方向:植物病毒学
  • 办公地点:农学楼605室
  • 办公电话:
  • 电子邮件:wxy5551@126.com

个人简介

学习经历:

1998年9月-2002年7月,东北农业大学,获农学学士学位;

2004年9月-2009年7月,云南农业大学,植物病理学,获农学博士学位;

作经历:

2007年4月-2009年7月,浙江大学生物技术所合作研究

2009年8月-2012年12月,浙江农林大学农业与食品科学学院,讲师

2012年12月-2017年12月,浙江农林大学农业与食品科学学院,副教授

2018年1月-至今,东北农业大学农学院,副教授

教学工作

讲授《微生物》、《普通植物病理学》、《农业植物病理学》、《植物病毒学》《环境污染与食品安全》、《农业微生物》、《植物保护基础实习》、《植物保护综合实习》本科生课程;讲授《高级植物病理学研究方法》、《有害生物分类鉴定与监测》、《植物保护技术与应用(案例课)》《SCI科技论文写作》研究生课程

科研项目

1、大豆抗苜蓿花叶病毒种质资源筛选与鉴定(LH2019C027),黑龙江省自然科学基金联合引导项目,2019年7月-2021年7月;

2、植物病毒抑制寄主系统性获得免疫的分子机理及应用研究(ZD2018002),黑龙江自然科学基金重点项目,2018年7月-2021年7月,(参加,第二);

3、大豆抗苜蓿花叶病毒资源鉴定与抗性基因定位研究,东北农业大学第四批“学术骨干”项目,2018年10月-2020年10月;

4、烟草10个基因的克隆、载体构建与遗传转化,贵州烟草研究院,2016年11月-2017年12月;

5、国家留学基金委出国留学项目([2014]3012),国家留学基金委,2015年10月-2016年10月;

6、利用人工ta-siRNA表达体系在水稻中进行多价抗病毒的研究,国家自然科学基金青年基金项目(31101415),2012年1月-2014年12月;

7、基于拟南芥TAS基因的ta-siRNA表达载体的构建与抗病毒评价(Y3110277),浙江省自然科学基金一般项目,2010年1月-2012年12月;

8、桑树雌雄植株MMLRaV侵染相关mal-miR01的功能分析(Q17C160011),浙江省自然科学基金2016.10-2019.12,(参加,第三);

9、小麦叶枯病菌致病相关基因的分离与鉴定(Y3110316),浙江省自然科学基金,2012-2014,(参加,第三);

10、2010-2012番茄环斑病毒传播介体的确定及其防治策略的研究,浙江农林大学校科研发展基金,0.5;

11、灰霉病菌啶酰菌胺抗药性突变体对甲氧基丙烯酸酯类杀菌剂表现负交互抗药性的机制(31071711),国家自然科学基金面上项目,2011-2013,(参加,第二);

12、浙江省蔬菜主要病害抗药性的普查、监测及治理技术开发,浙江省科技厅公益性项目,2011-2013,(参加,第二);

13、浙江农林大学人才启动基金,2009

论文著作

1.Xue Jiang, Yameng Luan, Mengzhu Chai, Yingshuai Yang, Yuting Wang,Wenjia Deng, Yonggang Li, Xiaofei Cheng*, Xiaoyun Wu*. The N-terminal α-helix of potato virus X-encoded RNA-dependent RNA polymerase is required for membrane association and multimerization. Viruses, 2022, 14: 1907.

2.Xiaoyun Wu, Mengzhu Chai, Jiahui Liu, Xue Jiang, Yingshuai Yang, Yushuang Guo, Yong Li*, Xiaofei Cheng*. Turnip mosaic virus manipulates DRM2 expression to regulate host CHH and CHG methylation for robust infection. Stress Biology, 2022, 2: 29.

3.Xiaofei Cheng, Yameng Luan, Xiaoyun Wu. Plant nonhost resistance against viruses: current status and future prospects. In: Guar RK, Khurana SMP, Sharma P, Hohn T eds. Plant virus-host interaction-molecular approaches and viral evolution (second edition). Academic press. London, UK. 2021, 45-57.

4.孙婷,王馨玥,刘洪义,程晓非,武晓云. 马铃薯奥大古巴花叶病毒RT-PCR检测方法的建议及应用. 植物检疫,2021,35(3):47-52.

5.Xiaoyun Wu, XiaofeiCheng*. Intercellular movement of plant RNA viruses: Targeting replicationcomplexes to the plasmodesma for both accuracy and efficiency. Traffic, 2020, 21(12): 725-736.

6.Xinyang Che, Xue Jiang,Xinlei Liu, Xiaoyan Luan, Qi Liu, Xiaofei Cheng*, Xiaoyun Wu*. First report ofAlfalfa mosaic virus on soybean in Heilongjiang, China. Plant Disease, 2020,104(11): 3085.

7.Xiaoyun Wu, Jiahui Liu,Xiaofei Cheng*. First report of Camellia japonica associated emaravirus 1associated with camellia leaf ringspot and flower color-breaking disease inChina. Plant Disease, 2020, 104(12): 3271.

8.Mengzhu Chai1, XiaoyunWu1, Jiahui Liu, Yue Fang, Yameng Luan, Xiaoyan Cui, Xueping Zhou, Aiming Wang,Xiaofei Cheng*. P3N-PIPO interacts with P3 via the shared N-terminal domain torecuruit viral replication vesicles for cell-to-cell movement. Journal ofVirology, 2020, 94(8): e01898-19

9.Ruonnan Zhang+, Xiaoyun Wu +, Xue Jiang, Xiaoxia Wu, Xiaoyan Luan, Xiaofei Cheng*. Molecular characterization of Common bean curly stunt virus: a novel recombinant geminivirus in China. Archives of Virology, 2019, DOI: 10.1007/ s00705-019-04473-w.

10.Jiahui Liu, Lili Zhang, Feifei Xu FF, Mengzhu Chai, Xiaodong Wu, Unsong Kim, Dianqiu Lv,Xiaoyun Wu, Xiaoxia Wu, Xiaofei Cheng*. Molecular analysis of a divergent isolate of potato virus H from potato reveals novel evolutionary feature of Carlaviruses. Canadian Journal of Plant Pathology, 2019, DOI: 10.1080/07060661.2019.1625947.

11.Xiaoyun Wu, Adrian Valli, Juan Antonio Garcia, Xueping Zhou, Xiaofei Cheng*. The tug-of-war between plants and viruses: great progress and many remaining questions. Viruses, 2019, 11: 203

12.Xiaoyun Wu, Jiahui Liu, Chai Mengzhu, Jinhui Wang, Dalong Li, Aiming Wang, Xiaofei Cheng*. The potato virus X TGBp2 protein plays dual functional roles in viral replication and movement. Journal of Virology, 2018, doi:10.1128/JVI.01635-18.

13.Xiaoyun Wu, Qi Liu, Chai Mengzhu, Lili Zhang, Xiaofei Cheng*, First report of Potato aucuba mosaic virus on potato in China. Plant Disease, 2018 102(12): 2671.

14.Xiaodong Wu,Xiaoyun Wu, Wenbin Li, Xiaofei Cheng*. Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China. Archives of Virology, 2018, DOI: 10.1007/s00705-018-3743-8.

15.Xiaoyan Cui, Hoda Yaghmaiean, Guanwei Wu,Xiaoyun Wu, Bin Chen, Thorn G, Aiming Wang. The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Virology, 2017, 510: 147-155

16.蔡健宇,武晓云,郭玉双,李翔宇.烟草基因组中AGO蛋白的生物信息学分析,江西农业学报,2017,29(2):1-8.

17.詹琳琳,郭玉双,蔡健宇,武晓云,杨静. NtMAP70基因在烟草抗马铃薯Y病毒过程中的作用,福建农林大学学报(自然科学版),2016,45 (5) :556-560.

18.Linyun Chen, Xiaofei Cheng, Jianyu Cai, Linlin Zhan, Xiaoxia Wu, Qi Liu,Xiaoyun Wu*. Multiple virus resistance using artificial trans-acting siRNAs. Journal of Virological Methods, 2016, 228: 16–20.

19.Xiaofei Cheng, Fangfang Li, Jianyu Cai, Wei Chen, Nan Zhao, Yuqiang Sun, Yushuang Guo, Xiulin Yang,Xiaoyun Wu*. Artificial TALE as a convenient protein platform for engineering broad-spectrum resistance to begomoviruses. Viruses, 2015, 7: 4772-4782.

20.Yilun Wang, Xiaofei Cheng, Xiaoxia Wu, Aiming Wang,Xiaoyun Wu*. Characterization of complete genome and small RNA profile of pagoda yellow mosaic associated virus, a novel badnavirus in China. Virus Research, 2014, 188: 103-108.

21.Wei Chen, Yongsheng Qian,XiaoynWu, Yuqiang Sun,XiaoxiaWu,XiaofeiCheng*. 2014. Inhibitting replication of begomoviruses using artificial zinc finger nucleases that target viral-conserved nucleotide motif. Virus Genes, 48: 494-501.

22.Xiaofei Cheng, Nasar Virk, Wei Chen, Shuqing Ji, Shuxian Ji, Yuqiang Sun,Xiaoyun Wu*. CpG usage in RNA viruses: data and hypotheses. Plos One, 2013, 8(9): e74109.

23.XiaofeiCheng,XiaoyunWu,HuizhongWang,YuqiangSun,YongshengQian, Luo Lu. 2012. High codon adaptation in citrus tristeza virus to its citrus host. Virology Journal, 9: 113.

24.Xiaoyun Wu, Haitao Ding, Liping Ke, Yueyong Xin, Xiaofei Cheng. Characterization of an acid-resistant glucose 1-dehydrogenase from Bacillus cereus var. mycoides, Romanian Biotechnological Letters, 2012, 17(4): 7540-7548.

25.Xiaofei Cheng, Jianxiang Wu, Xinying Ma, Huafu Fu,Xiaoyun Wu*. First report of citrus tristeza virus in Citrus changshanensis cv. Huyou in Zhejiang, China. Journal of plant Pathology, 2011, 93(s4): 68.

26.武晓云,刘琦,郭玉双,武小霞.2011.胡柚上柑橘衰退病毒的分子检测.东北农业大学学报,42(7):117-121.

27.Xiaoyun Wu, Jianxiang Wu, Xiaofei Cheng*. 2010. First report of natural infection ofBroad bean wilt virus-2onLactuca indica. Journal of Plant Pathology, 92(s4): 119.

28.Xiaoyun Wu, Xueping Zhou,ZhengyueLi,WennanGao,JianxiangWu*. 2009. Monoclonal antibodies againstTobacco rattle virusand its application in the detection of TRV isolates. Indian Journal of Virology, 20(2): 64-69.

29.Wu XY, Zhang HR, Li ZY. 2009. Occurrence and seasonal pattern of western flower thrips,Frankliniella occidentalis(Pergande) (Thysanoptera: Thripidae) in Yunnan Province. Acta Phytopathologica et Entomologica Hungarica, 44(1): 135-146.

30.武晓云,程晓非,张仲凯,桂富荣,李正跃.2009.西花蓟马(Frankliniella occidentalis)rDNA ITS2和CO I基因5’末端序列的克隆与比较分析.浙江大学学报(农业与生命科学版),35(4):355-364.

其他成果

专利:

  1. 一种苜蓿花叶病毒的侵染性克隆、重组农杆菌及构建方法(排名第2),ZL202010461785.8,2018。

  2. 一种马铃薯X病毒弱毒疫苗及其制备方法与应用(排名第2),ZL201811376177.6,2018。

  3. 检测烟草脆裂病毒的单克隆抗体细胞株和检测试剂盒(排名第3),ZL200810225905,2012

  4. 蓟马昆虫室内饲养罐(排名第3),ZL200520100047,2007。