近期，国际学术期刊《International Journal of Molecular，（IF= 6.208）在线发表了东北农业大学农学院董守坤团队题为“Transcriptomic and Metabolomic Analysis of Seedling-Stage Soybean Responses to PEG-Simulated Drought Stress”的研究论文。
东北农业大学农学院董守坤团队以抗旱性较强的大豆品种“黑农44”和抗旱性较弱的“黑农65”为材料，用15%（w/v）聚乙二醇（PEG-6000）模拟干旱胁迫，采用RNA测序和高效液相色谱串联质谱技术研究了干旱胁迫下大豆幼苗期叶片转录组和代谢组的变化，分析了两个大豆品种的基因表达和代谢物积累。结果表明，两个品种分别有4790个和3483个差异表达基因，以及156个和 124 个差异表达代谢物。综合分析发现，LOC100802571、LOC100814585、LOC100777350、LOC100787920、LOC100800547和LOC100785313等候选基因在两个品种之间表现出不同的表达趋势，这可能是“黑农44”和“黑农65”出现抗旱性差异的原因。此外，研究人员发现大量黄酮类化合物在干旱胁迫后表达上调，还鉴定出参与异黄酮生物合成和三羧酸循环的几个关键候选基因和代谢物，这些结果表明上述代谢途径在大豆干旱胁迫中具有十分重要的作用。
本研究剖析了大豆苗期的抗旱机理，鉴定的相关候选基因可为大豆抗旱育种提供思路。
Int. J. Mol. Sci., 20 Jun 2022
Transcriptomic and Metabolomic Analysis of Seedling-Stage Soybean Responses to PEG-Simulated Drought Stress
Author
Xiyue Wang, Shuang Song, Xin Wang, Jun Liu, Shoukun Dong*
*：College of Agriculture, Northeast Agricultural University, China
Abstract
Soybean is an important crop grown worldwide, and drought stress seriously affects the yield and quality of soybean. Therefore, it is necessary to elucidate the molecular mechanisms underlying soybean resistance to drought stress. In this study, RNA-seq technology and ultra-performance liquid chromatography–tandem mass spectrometry were used to analyze the transcriptome and metabolome changes in soybean leaves at the seedling stage under drought stress. The results showed that there were 4790 and 3483 DEGs (differentially expressed genes) and 156 and 124 DAMs (differentially expressed metabolites), respectively, in the HN65CK vs. HN65S0 and HN44CK vs. HN44S0 comparison groups. Comprehensive analysis of transcriptomic and metabolomic data reveals metabolic regulation of seedling soybean in response to drought stress. Some candidate genes such as LOC100802571,LOC100814585, LOC100777350 and LOC100787920, LOC100800547, and LOC100785313 showed different expression trends between the two cultivars, which may cause differences in drought resistance. Secondly, a large number of flavonoids were identified, and the expression of Monohydroxy-trimethoxyflavone-O-(6″-malonyl) glucoside was upregulated between the two varieties. Finally, several key candidate genes and metabolites involved in isoflavone biosynthesis and the TCA cycle were identified, suggesting that these metabolic pathways play important roles in soybean response to drought. Our study deepens the understanding of soybean drought resistance mechanisms and provides references for soybean drought resistance breeding.
